LOCUS       pLV-[hsa-miR-1204]        9121 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7042..7902
                     /label=Amp(R)
     promoter        8103..8643
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5647..5881
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6456..6911)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5953..6083
                     /label=SV40 pA
     misc_feature    6084..6297
                     /label=SV40 ori
     misc_feature    4968..5564
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3586
                     /label=intron
     CDS             3633..4958
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3376
                     /label=hsa-mir-1204 precursor
BASE COUNT     2167 a      2361 c      2479 g      2114 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca tcctcagaac 
     3121 cctgagtgga actttctaca ggatttatta ggagaaaaac cttcccggaa gctgcagaag 
     3181 gacaaataca gaatccgtgt ctgggagaaa cctcgtggcc tggtctccat tatttgagat 
     3241 gagttacatc ttggaggtga ggacgtgcct cgtggtctaa agcttcggca caagggccca 
     3301 actggaattc cacttacggg tatgactgtg gggtatatgc tgtacccatt gaattcccag 
     3361 agggatttgt taagtggcta gctcgagctt ttggagtacg tcgtctttag gttgggggga 
     3421 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc 
     3481 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat 
     3541 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaaaact 
     3601 acccctctag agtcgagcta ccggtcgcca ccatggtgag caagggcgag gaggataaca 
     3661 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc 
     3721 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 
     3781 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt 
     3841 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc 
     3901 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 
     3961 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc 
     4021 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg 
     4081 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc 
     4141 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga 
     4201 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca 
     4261 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg 
     4321 gcatggacga gctgtacaag gacccaccgg tcgccaccat gaccgagtac aagcccacgg 
     4381 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg 
     4441 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg 
     4501 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 
     4561 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 
     4621 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 
     4681 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 
     4741 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 
     4801 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 
     4861 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc 
     4921 gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgagc ggccgcgtcg acaatcaacc 
     4981 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 
     5041 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 
     5101 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 
     5161 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 
     5221 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 
     5281 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 
     5341 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 
     5401 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 
     5461 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 
     5521 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta 
     5581 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 
     5641 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact 
     5701 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
     5761 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
     5821 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
     5881 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 
     5941 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 
     6001 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 
     6061 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc 
     6121 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     6181 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     6241 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact 
     6301 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 
     6361 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 
     6421 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag 
     6481 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 
     6541 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 
     6601 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 
     6661 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 
     6721 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 
     6781 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 
     6841 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 
     6901 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 
     6961 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 
     7021 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 
     7081 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 
     7141 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 
     7201 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 
     7261 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 
     7321 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 
     7381 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 
     7441 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 
     7501 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 
     7561 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 
     7621 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 
     7681 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 
     7741 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 
     7801 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 
     7861 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 
     7921 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 
     7981 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 
     8041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 
     8101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 
     8161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 
     8221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 
     8281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 
     8341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 
     8401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 
     8461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 
     8521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 
     8581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 
     8641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 
     8701 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 
     8761 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 
     8821 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 
     8881 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 
     8941 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 
     9001 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat 
     9061 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg 
     9121 c 
//
